sucrose fraction (Thermo Fisher)

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Thermo Fisher sucrose fraction
Sucrose Fraction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sucrose+fractions/Sucrose/bio_rxiv__64898__2026__01__16__699988-293-3-13
Average 97 stars, based on 1 article reviews

sucrose fraction - by Bioz Stars, 2026-10

97/100 stars

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Isolation:

Article Title: T-Cell-Derived Exosomes From Multi Core Granules Exhibit Superior Caspase-3-Mediated Tumor-Suppressive Activity Compared to Those From Multivesicular Bodies.
Article Snippet: .. For isolated organelles and exosomes, protein concentrations in sucrose fractions were quantified using Pierce 660 nm Protein Assay reagent. .. For isolated organelles and exosomes, protein concentrations in sucrose fractions were quantified using Pierce 660 nm Protein Assay reagent.

Article Title: Targeting oncogenic activation of FLT3/SREBP/FASN promotes the therapeutic effect of quizartinib involving disruption of mitochondrial phospholipids.
Article Snippet: .. Total RNA from sucrose fractions was isolated by TRIzol reagent (Thermo) and subjected to real-time qPCR analysis. ..

Article Title: Targeting oncogenic activation of FLT3/SREBP/FASN promotes the therapeutic effect of quizartinib involving disruption of mitochondrial phospholipids
Article Snippet: .. Total RNA from sucrose fractions was isolated by TRIzol reagent (Thermo) and subjected to real-time qPCR analysis. ..

Article Title: T‐Cell‐Derived Exosomes From Multi Core Granules Exhibit Superior Caspase‐3‐Mediated Tumor‐Suppressive Activity Compared to Those From Multivesicular Bodies
Article Snippet: .. For isolated organelles and exosomes, protein concentrations in sucrose fractions were quantified using Pierce 660 nm Protein Assay reagent. .. For isolated organelles and exosomes, protein concentrations in sucrose fractions were quantified using Pierce 660 nm Protein Assay reagent.

Purification:

Article Title: GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation
Article Snippet: For RNaseA treatments, RNaseA (ThermoFisher Scientific) was added to the cell lysate at a final concentration of 600 U/mL at the stage of protein sample preparation, and sucrose gradients were prepared as described. .. Total RNA was extracted from the whole cells and sucrose fractions using the GeneJET RNA purification kit (K0731; Thermo Fisher Scientific) and Trizol reagent (Invitrogen), respectively. .. Single-stranded cDNA was synthesized from extracted mRNA with the Maxima first strand cDNA synthesis kit (K1671; Thermo Fisher Scientific).

Article Title: GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation
Article Snippet: For RNaseA treatments, RNaseA (ThermoFisher Scientific) was added to the cell lysate at a final concentration of 600 U/ml at the stage of protein sample preparation, and sucrose gradients were prepared as described. .. Total RNA was extracted from the whole cells and sucrose fractions using the GeneJET RNA purification kit (K0731; Thermo Fisher Scientific) and Trizol reagent (Invitrogen), respectively. .. Single-stranded cDNA was synthesized from extracted mRNA with the Maxima first strand cDNA synthesis kit (K1671; Thermo Fisher Scientific).

Article Title: GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation.
Article Snippet: For RNaseA treatments, RNaseA (ThermoFisher Scientific) was added to the cell lysate at a final concentration of 600 U/mL at the stage of protein sample preparation, and sucrose gradients were prepared as described. .. Total RNA was extracted from the whole cells and sucrose fractions using the GeneJET RNA purification kit (K0731; Thermo Fisher Scientific) and Trizol reagent (Invitrogen), respectively. .. Single-stranded cDNA was synthesized from extracted mRNA with the Maxima first strand cDNA synthesis kit (K1671; Thermo Fisher Scientific).

Real-time Polymerase Chain Reaction:

Article Title: Targeting oncogenic activation of FLT3/SREBP/FASN promotes the therapeutic effect of quizartinib involving disruption of mitochondrial phospholipids
Article Snippet: .. Total RNA from sucrose fractions was isolated by TRIzol reagent (Thermo) and subjected to real-time qPCR analysis. ..

cDNA Synthesis:

Article Title: Model-independent reorganization of translation in TDP-43 Amyotrophic Lateral Sclerosis
Article Snippet: .. Equal volumes of RNA from sucrose fractions were used for cDNA synthesis using the RevertAid First Strand cDNA synthesis kit (Thermo Fisher Scientific). .. For total cytoplasmic RNA 500 ng were used for cDNA synthesis using the RevertAid First Strand cDNA synthesis kit (Thermo Fisher Scientific).

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